Selenite broth was originated by leifson, while observing good recovery of salmonella spp. Selenite broth is recommended as enrichment media for the isolation of salmonellae from faeces, urine or other pathological materials. Summary and explanation selenite broth was devised by leifson,1 who demonstrated that selenite was inhibitory for coliforms and certain other microbial. Selenite cystine broth intended use selenite cystine broth is used as a selective enrichment medium for the isolation of salmonella from feces, foods, water and other materials of sanitary importance. Composition of selenite f broth casein enzymic hydrolysate 5 gml lactose 4 gml sodium phosphate 10 gml sodium hydrogen selenite nahseo3 4. New selenite selective enrichment medium for the isolation of typhoid and paratyphoid bacilli. Selenite f broth culture media online microbiology notes. It contains watersoluble substances of animal tissue, which include carbohydrates, organic nitrogen compounds, water soluble vitamins, and. Selenite cystine broth for microbiology sigmaaldrich.
Bacterial culture media can be classified on the basis of composition, consistency, and purpose classification of bacterial culture media on the basis of consistency solid medium. Summary and explanation leifson found that selenite inhibited fecal streptococci and coliforms during the first 812 hours of incubation, thereby. The relative effectiveness of rappaportvassiliadis rv medium, selenite cystine sc broth, and tetrathionate tt broth for the recovery of salmonella spp. Selenite broth selenitef broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Remel selenite cystine broth is selective enrichment broth for isolation of salmonella spp. Sodium biselenite acts as the selective agent, while lactose is added as a fermentable carbohydrate to prevent a rise in ph value during incubation because any increase in ph will reduce the selective activity of selenite. Selenite lactose broth is used for enrichment culture of salmonellae from feces, food products, water and other materials. Selenite f broth eb02e typical formula grams per litre peptone 5. Characteristics of the components used in nutrient agarbroth. Sodium hydrogen selenite or detecting faecal coliforms drinking in water waste water, seawater and foods samples by mpn method. Technical information selenite broth selenite f broth twin pack product code. It is the most widely used enrichment medium to isolate salmonella typhi from fecal specimen of suspected patients andor from carriers during salmonella outbreaks. Further study of strontium selenite and selenite f broths.
Selenite broth selenite f broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Silver selenite, an inorganic compound of formula ag 2 seo 3. Composition ingredients gramslitre ingredients gramslitre part a peptic digest of animal tissue 5. Selenite f broth principle, uses, preparation and result interpretation. The efficiency of strontium selenite and selenite f broths in the isolation of s. Nutrient broth contains same ingredients except agar. Selenite mannitol broth is an alternative to selenite f broth for the selective enrichment of salmonellae spp from clinical, food and environmental specimens. A selenium compound with the molecular formula h2so3. Chemtrec usa 8004249300 safety data sheet r454582 supplier oxoid limited wade road basingstoke. Shop online for a wide selection of bd bbl mycoflask selenitef broth prepared media for isolation of salmonella from feces, urine, water, foods and other materials of sanitary. Selenite broth is used as an enrichment medium for the isolation of salmonella and shigella from stool, urine, water and food products.
Selenite f broth principle, uses, preparation and result. Selenitef broth was devised by leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in. A small amount of colored precipitate is not detrimental. At the same time, the number of false positive isolates accruing to strontium selenite broth. Solid medium contains agar at a concentration of 1. A seleniumcontaining anion or ionic compound with the seo 3 2.
Selective enrichment of salmonella from foods format. Not all products are available for sale in all territories. Selenite f broth twin pack m025s recommended is used as an enrichment medium for isolation of shigella from food samples. Mccance, in laboratory methods in microbiology, 1966. F in selenite f broth is faeces, because it is mostly used for the isolation of salmonella and shigella from faecal specimens. Sodium selenite is the inorganic compound with the formula na 2 seo 3. Beef extract is an aqueous extract of lean beef tissues. Subcultures should be made from the top of the broth after not more than 24 hours incubation as after this time there is. Selenite broth is used as a selective medium for the isolation of salmonella species.
Remel selenite cystine broth thermo fisher scientific. Selenite f broth is the medium used for the selective enrichment of salmonella spp from both clinical and food samples. Selenous acid, the conjugate acid, with the formula h 2 seo 3. Selenite broth is used as a selective enrichment for the cultivation of salmonella spp. The fermentation of mannitol by salmonellae is said to correct the alkaline ph swing which can occur during incubation. Criterion selenite cystine broth for the detection of. A medium for the selective enrichment of salmonella spp from both clinical and food samples.
Enzymatic digest of casein and animal tissue supply nitrogenous substances and carbohydrates for bacterial growth. Selenite broth was developed by leifson for enrichment of salmonella from sources such as feces, urine, and food. If possible even larger samples are preferable with proportionately larger amounts of the enrichment broths being used. They recommended the use of this principle for the examination of sewage and river water containing large numbers of. The pentahydrate na 2 seo 3 h 2 o 5 is the most common watersoluble selenium compound. Selenite broth was originated by leifson, while observing good recovery of. Dulcitol selenite broth selenitef broth w dulcitol. It is a buffered lactose peptone broth to which sodium biselenite is added as the selective agent. This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.
Composition ingredients gms litre part a tryptone 5. Handbook of media for clinical and public health microbiology. Selenite broth selenitef broth laboratoires humeau. Summary and explanation selenite broth was devised by leifson,1 who demonstrated that. Selenite broth selenitef broth is used as an enrichment medium buffered with lactose peptone broth to which sodium biselenite is added as the selective agent for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Remel and oxoid products are now part of the thermo scientific brand, combining powerful manual, semiautomated and fully automated test. Tryptone atau polypeptone 5 g lactose 4 g sodium acid selenite nahso4 4 g na2hpo4 10 g lcystine 0,01 g aquadest 1 liter panaskan sampai mendidih agar melarut. Sodium selenite bioreagent, suitable for cell culture catalog number s5261 storage at room temperature cas rn 10102188 synonym. Selenite f broth is recommended as an enrichment medium for the isolation of salmonella species from faeces, urine or other pathological materials in accordance with indian pharmacopoeia. Selenite broth is used as a selective enrichment for the cultivation of. Recommended as enrichment media for the isolation of salmonellae from faeces, urine or other pathological materials. Reduce media preparation time and simultaneously enrich and isolate salmonella spp. Selenite cystine broth is an internationally accepted medium see icmsf, 1978.
Page 1 of 7 sds 7155 rev 0 june 2016 section 1 identification of the substancemixture and of the company 1. Product summary and explanation the growth and isolation of salmonella in food and other samples can be suppressed from foodprocessing procedures including exposure to low and or high temperatures, drying, radiation, preservatives, and. Can we use selenite f broth media to revive organisms like pseudomonas aeruginosa, staphylococcus aureus, micrococcus spp. Pi7283, rev 05, march 2008 selenite cystine broth 7283 intended use selenite cystine broth is used for the selective enrichment of salmonella species. Based on buffered mannitol broth, it is made selective by the addition of sodium biselenite. Section 1 identification of the substancemixture and of. It used as a source of selenium, especially for patients. Tm2810m mannitol selenite broth 10ml 16x125mm screw cap tube 10pkg tm2815l mannitol selenite broth 15ml 20x150mm screw cap tube 10pkg references 1. Selenite f broth is widely used as an enrichment medium for the.
Add aseptically 10 g of the food product after thorough mixing of the sample to 100 ml of selenite broth and add a further 10 g to 100 ml of tetrathionate broth. Selenitef broth is an enrichment medium for the isolation of salmonella and some species of shigella. Intended use hardy diagnostics criterion selenite cystine broth is a selective enrichment for isolation of salmonella spp. Withdraw approximately 1ml of the supernatant and inoculate 10 ml of selenite broth. Selenite broth selenite f broth twin pack m052 intended use.
It is recommended by bis committee composition ingredients gms litre part a peptone 5. A rise in ph decreases selective activity of selenite. Can we use selenite f broth media to revive organisms like. Ivesons revised formula personal communication as follows. Of these, 96 76% were isolated with selenite f broth and 118 94% were isolated with strontium selenite broth. Selenite cystine broth is a modifica tion north and bartram, 1953 of the original leifson 1936 formula, differing only in the addition of lcystine which is considered to enhance salmonella growth by reduction of toxicity. Rv medium made from its individual ingredients and incubated at 42 degrees c was compared with a commercial preparation of sc broth. Casein enzymic hydrolysate provides nitrogenous substances and other essential ingredients.
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